Abstract
In modern practical medicine, along with pharmacological agents, more and more attention is paid to physiotherapeutic methods. Over the past decades, extremely high-frequency therapy (EHF-therapy) has been widely used. The electromagnetic radiation of the EHF range is used in medicine not only as a therapeutic, but also as a diagnostic tool. At the moment, there are various methods for determining individual sensitivity to EHF-therapy at the cellular level of patients with cardiovascular diseases, although this method of physiotherapy is also successfully used to treat neurological diseases. A cytobiophysical technique was developed and studied to determine individual sensitivity to EHF-therapy in neurology. The technique is based on determining the number of negatively charged nuclei of the patient’s buccal epithelium cells before and after exposure to cells of electromagnetic radiation from the EHF range and comparing the obtained parameters to determine individual sensitivity to EHF therapy. The measurements were carried out using buccal epithelium of conditionally healthy donors 24–35 years old of both sexes (n=8), as well as patients with degree I and II degree discirculatory encephalopathy at the age of 41–77 years (n=55) who were hospitalized. A generator of type G4-141 served as a source of EHF radiation, irradiation was carried out at a wavelength of λ=7,1 mm, and a power flux density of 10 mW/cm2. The cytobiophysical technique allows us to solve the problem of individualization in the case of EHF-therapy, since the current approaches to the treatment of patients with discirculatory encephalopathy require maximum individualization of treatment and a combination of drug treatment with physiotherapeutic methods of treatment. The cytobiophysical technique is easy to use, non-invasive, painless for the patient, allows you to determine individual sensitivity to EHF-therapy for various pathologies, and also allows you to quickly (study time is 15–20 minutes) to monitor the effectiveness of any type of therapy, including EHF-therapy at all stages of treatment.
References
Ordynskaia T.A., Poruchikov P.V., Ordynskii V.F. (2008). Volnovaia terapiia [Wave therapy]. Мoscow, 496 p. [in Russian].
Holovacheva T.V., Kirichuk V.F., Ushakov V.Iu., Troitskii V.V., Semenova S.V. (1994). Primeneniie elektromahnitnykh voln millimetrovoho diapazona v kompleksnom lechenii bolnykh OIM [The use of millimeter-wave electromagnetic waves in the complex treatment of patients with acute myocardial infarction]. Sovremennyie problemy meditsinskoi nauki – Modern Problems of Medical Science. Arkhangelsk: Izdatelstvo SHMU, chapter 2, pp. 37–39 [in Russian].
Lebedeva A.Yu. (2002). Itohi i perspektivnyie primeneniia millimetrovykh voln v kardiolohii [Results and promising applications of millimeter waves in cardiology]. Millimetrovyie volny v biolohii i meditsine – Millimeter Waves in Biology and Medicine, № 1, pp. 21–24 [in Russian].
Istomina I.S. (2012). KVCh-terapiia v klinicheskoi praktike [EHF-therapy in clinical practice]. Fizioterapiia, balneolohiia i reabilitatsiia – Physiotherapy, balneology and rehabilitation, № 2, pp. 38–45 [in Russian].
Holant M.B., Savostianova N.A. (1989). Radioelektronnoie obosnovaniie vozmozhnosti ispolzovaniia KVCh-heneratorov, rabotaiushchikh na odnoi i toi zhe chastote, dlia lecheniia mnohikh zabolevanii [Radio-electronic substantiation of the possibility of using EHF generators operating at the same frequency for the treatment of many diseases]. Elektronnaia tekhnika. Seriia Elektronika SVCh – Electronic Equipment. Microwave Electronics Series, issue 6 (420), pp. 48–53 [in Russian].
Lebedeva N.N., Kotrovskaia T.I. (2003). Eksperimentalno-klinicheskiie issledovaniia v oblasti biolohicheskikh effektov millimetrovykh voln [Experimental and clinical research in the field of biological effects of millimeter waves]. Millimetrovyie volny v biolohii i meditsine – Millimeter Waves in Biology and Medicine, № 29, pp. 20–43 [in Russian].
Istomina I.S., Dovhaniuk A.P., Bizhek M.Kh. (2006). Primeneniie fizicheskikh faktorov v lechenii zabolevanii serdechno-sosudistoi sistemy [The use of physical factors in the treatment of diseases of the cardiovascular system]. Proceedings from Meditsinskaia reabilitatsiia bolnykh s patolohiei oporno-dvihatelnoi i nervnoi sistem: nauch.-prakt. konf. – Medical Rehabilitation of Patients with Pathology of the Musculoskeletal and Nervous Systems: scientific-practical. conf. Moscow, pp. 247–248 [in Russian].
Kirichuk V.F., Malinova L.I., Krenitskii A.P. et al. (2003). Hemoreolohiia i elektromahnitnoie izlucheniie KVCh-diapazona [Hemorheology and EHF electromagnetic radiation]. Saratov: SHMU, 126 p. [in Russian].
Malakhov V.A., Arkhipova Ye.A., Nosatov A.V., Fisun A.I. (2017). Ispolzovaniie elektromahnitnoho izlucheniia KVCh-diapazona v diahnostike i lechenii sosudistykh zabolevanii holovnoho mozha [The use of EHF electromagnetic radiation in the diagnosis and treatment of cerebrovascular diseases]. Kharkiv: Palmarium, 120 p. [in Russian].
Arkhypova K.A., Bilous O.I., Bryuzginova N.V., Fisun A.I., Malakhov V.O., Nosatov A.V. et al. (2015). Role of microwave radiation in self-blood therapy. Telecommunications and Radio Engineering, vol. 74, № 14, pp. 1305–1315.
Bryuzginova N.V., Bilous O.I., Sirenko S.P., Fisun A.I. (2018). Changes in electrokinetic indices of nuclei in buccal epithelium cells under the influence of millimeter-wave electromagnetic radiation. Telecommunications and Radio Engineering, vol. 77, № 3, pp. 263–268.
Avshalumov A.S., Filaretov H.F., Sudakov K.V. (2006). Novaia informatsionnaia tekhnolohiia sistemnoi diahnostiki normalnykh i patolohicheski izmenennykh orhanov cheloveka [New information technology for system diagnostics of normal and pathologically changed human organs]. Meditsinskaia tekhnika – Medical Equipment, № 3, pp. 13–18 [in Russian].
Bessonov A.Ye., Kalmykova Ye.A. (2012). Analizator-indikator millimetrovykh sihnalov s BAT kompiuterizirovannyi «AIS-LIDO» [Computer-based analyzer-indicator of millimeter-wave signals with BAT «AIS-LIDO»]. Millimetrovyie volny v biolohii i meditsine – Millimeter Waves in Biology and Medicine, № 2 (66). Retrieved from http://lido.su/nauka-informacionnaja-medicina/vrachu-/32/analizator-indikator-milimetrovovolnovyh-signalov-/.
Shakhbazov V.G., Shkorbatov Yu.G. (1994). Patent 2009494 C1 RU GO1 № 33/483. Sposob issledovaniia funktsionalnoho sostoianiia cheloveka [Patent 2009494 C1 RU GO1 № 33/483. A method of studying the functional state of a person]. Otkrytiia. Izobreteniia – Discoveries. Inventions, № 5 [in Russian].
Shkorbatov Yu.G., Shakhbazov V.G., Navrotskaya V.V. et al. (2002). Application of intracellular microelectrophoresis to analysis of the influence of the low-level microwave radiation on electro-kinetic properties of nuclei in human epithelial cells. Electrophoresis, vol. 23, № 13, pp. 2074–2079.
Shakhbazov V.G., Kolupaieva T.V., Nabokov A.L. (1986). Novyi metod opredeleniia biolohicheskoho vozrasta cheloveka [A new method for determining the biological age of a person]. Laboratornoie delo – Laboratory Science, № 7, pp. 404–406 [in Russian].
Betskii O.V., Lebedeva N.N. (2001). Sovremennyie predstavleniia o mekhanizmakh vozdeistviia nizkointensivnykh millimetrovykh voln na biolohicheskiie obiekty [Modern ideas about the mechanisms of the impact of low-intensity millimeter waves on biological objects]. Millimetrovyie volny v biolohii i meditsine – Millimeter Waves in Biology and Medicine, № 3, pp. 5–19 [in Russian].
Bilous O.I., Bryuzginova N.V., Sirenko S.P., Fisun A.I. (2016). Efficiency testing of biologically significant effects of millimeter waves. Telecommunications and Radio Engineering, vol. 75, № 8, pp. 757–762.
Deviatkov N.D., Holant M.B., Betskii O.V. (1991). Millimetrovyie volny i ikh rol v protsessakh zhiznedeiatelnosti [Millimeter waves and their role in the processes of life]. Мoscow: Radio i sviaz, 168 p. [in Russian].
Semenova A.K., Alekseieva O.P., Balchuhov V.A., Kornaukhov A.V., Anisimov S.I., Korkotashvili L.V. (2007). Patent 2 292 557 С1 RF, MPK G01N 33/84, A61N 5/02. Sposob prohnozirovaniia effektivnosti vozdeistviia nizkointensivnym elektromahnitnym izlucheniiem millimetrovoho diapazona u bolnykh serdechno-sosudistymi zabolevaniiami [A method for predicting the effectiveness of low-intensity electromagnetic radiation of the millimeter range in patients with cardiovascular diseases]. The VMI FSB of Russia, A. Semenova, O. Alekseeva (patent holder). № 2005113345/15, stated on 03.05.05; published on 27.01.07, Newsletter № 3 [in Russian].
Parshina S.S., Kirichuk V.F., Holovacheva T.V., Rebrova T.B., Hrekova N.D. (1993). Avtorskoie svidetelstvo 1832197A1 USSR, G 01 N 33/50. Sposob opredeleniia individualnoi chuvstvitelnosti k KVCh-terapii [Certificate of authorship. The method of determining individual sensitivity to EHF-therapy]. № 4903443/14; stated on 18.01.91, published on 07.08.93, Newsletter № 29 [in Russian].
Holovacheva T.V., Parshina S.S., Nikolenko V.N., Chernenkov Yu.V., Afanasieva T.N. (2012). Saratovskaia kardiolohicheskaia shkola KVCh-terapii: istoriia razvitiia, dostizheniia, perspektivy [Saratov cardiology school of EHF-therapy: history of development, achievements, prospects]. Proceedings from Elektromahnitnyie neioniziruiushchiie izlucheniia maloi moshchnosti v klinicheskoi praktike: internet-konferentsiia s mezhdunarodnym uchastiiem – Low-power electromagnetic non-ionizing radiation in clinical practice: an online conference with international participation. Retrieved from https://medconfer.com/node/1590 [in Russian].
Betskii O.V., Deviatkov N.D., Kislov V.V. (1996). Millimetrovyie volny nizkoi intensivnosti v meditsine i biolohii [Low intensity millimeter waves in medicine and biology]. Zarubezhnaia radioelektronika – Foreign Electronics, № 12, pp. 3–15 [in Russian].
Lukianov V.F., Afanasieva T.N., Romanova O.V. et al. (1991). Primeneniie KVCh terapii pri lechenii razlichnykh patoheneticheskikh variantov hipertonicheskoi bolezni [The use of EHF therapy in the treatment of various pathogenetic variants of hypertension]. Proceedings from Millimetrovyie volny v meditsine: sbornik materialov Ros. simpoziuma s mezhdunarodnym uchastiiem – Millimeter waves in medicine: a collection of materials of the Russian symposium with international participation, vol. 1, pp. 71–75 [in Russian].
Nosatov A.V. (2018). Optymizatsiia likuvannia dystsyrkuliatornykh entsefalopatii autohemoterapiieiu nadvysokochastotno modyfikovanoiu kroviu [Optimization of the treatment of dyscirculatory encephalopathies by autohemotherapy with ultra-high frequency modified blood]. Extended abstract of candidate’s thesis. Kharkiv, 20 p. [in Ukrainian].
Bilous O.I., Malakhov V.A., Nosatov A.V., Sirenko S.P., Fisun A.I. (2009). Patent na vynakhid № 90704 Ukraina, N0R7/00, A61K41/00. Prystrii dlia oprominiuvannia biolohichnykh ridyn elektromahnitnymy khvyliamy milimetrovoho diapazonu [Patent for invention № 90704 Ukraine, Н0Р7/00, А61К41/00. Device for irradiation of biological fluids by millimeter-wave electromagnetic waves]. O.Ya. Usikov Institute for Radio Physics and Electronics of the National Academy of Sciences of Ukraine (applicant and patent holder). № а2007 10113, stated on 10.09.07, published on 10.03.09, Newsletter № 5 [in Ukrainian].
Belous O.I., Fisun A.I., Malakhov V.A., Nosatov A.V., Sirenko S.P. (2008). EHF-autohemotherapy. Telecommunications and Radio Engineering, vol. 67, issue 10, pp. 867–874.
Seliverstov R.Yu., Hurchin A.F., Tyshkevich T.H., Hurskaia O.Ye. (2013). Patent № 0002484765 RF, MPK A61B5/0476 A61N5/02. Sposob kontrolia sostoianiia bolnoho entsefalopatiei pri KVCh-terapii [Patent № 0002484765 of the Russian Federation, IPC A61B5/0476 A61N5/02. A method for monitoring the condition of a patient with encephalopathy with EHF therapy]. Seliverstov R.Yu., Hurchin A.F., Tyshkevich T.H., Hurskaia O.Ye. (applicant and patent holder). № 2011111655/14, stated on 28.03.11, published on 20.06.13, Newsletter № 17 [in Russian].