Keywords
NO-synthase
arginase
How to Cite
Abstract
The article presents data on the state of lipid peroxidation and the activity of NO-synthase isoforms and arginase in lymphocytes and peripheral blood serum in men injured in combat (bullet and shrapnel wounds). 68 men injured as a result of hostilities (shrapnel and bullet wounds), who made up the main group, were examined. 48 practically healthy men were included in the control group. Patients of the main and control groups were divided into two age subgroups each: 20–39 years and 40–60 years. The MDA content in the blood serum of patients of both subgroups of the main group was 1.4 times higher than in the individuals of the corresponding subgroups of the control group (p<0.05). In peripheral blood lymphocytes, the MDA content in patients of both age subgroups of the main group was 1.3–1.4 times higher than in individuals of the corresponding subgroups of the control group (p<0.05; p<0.01). The activity of inducible (Ca2+-independent) de novo synthesis of NO significantly increases in blood serum and lymphocytes of men injured as a result of hostilities. At the same time, the activity of Ca2+-dependent NO-synthase in blood lymphocytes of men injured as a result of hostilities was 2.4 times lower compared to the values in practically healthy men (p<0.001), while it almost did not change in blood serum. It was confirmed that the inducible NOS can activate the processes of lipid peroxidation in men injured as a result of hostilities, as a direct significant correlation of high strength was recorded between the activity of Ca2+-independent iNOS and the MDA content (r=0.74; p<0.05). A significant correlation of medium strength was also established between arginase activity and MDA content (r=0.52; p<0.05).
Keywords: malondialdehyde, NO-synthase, arginase.
References
Agarwal A, Henkel R, Sharma R, Tadros NN, Sabanegh E. Determination of seminal oxidation-reduction potential (ORP) as an easy and cost-effective clinical marker of male infertility. Andrologia. 2018;50(3). DOI: 10.1111/and.12914. PMID: 29057493.
Chaudhary P, Janmeda P, Docea AO, Yeskaliyeva B, Abdull Razis AF, Modu B, et al. Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases. Front Chem. 2023;11:1158198. DOI: 10.3389/fchem.2023.1158198. PMID: 37234200.
Fafula R, Melnyk О, Gromnatska N, Vorobets D, Fedorovych Z, Besedina A, Vorobets Z. Prooxidant-antioxidant balance in seminal and blood plasma of men with idiopathic infertility and infertile men in combination with rheumatoid arthritis. Studia Biologica. 2023;17(2):15-26. DOI:10.30970/sbi.1702.719.
Hosseinzadeh Colagar A, Karimi F, Jorsaraei SG. Correlation of sperm parameters with semen lipid peroxidation and total antioxidants levels in astheno- and oligoasheno- teratospermic men. Iran Red Crescent Med J. 2013;15(9):780-5. DOI: 10.5812/ircmj.6409. PMID: 24616785.
Ko EY, Sabanegh ES Jr, Agarwal A. Male infertility testing: reactive oxygen species and antioxidant capacity. Fertil Steril. 2014;102(6):1518-27. DOI: 10.1016/j.fertnstert.2014.10.020. PMID: 25458618.
Akimov OY, Kostenko VO. Functioning of nitric oxide cycle in gastric mucosa of rats under excessive combined intake of sodium nitrate and fluoride. Ukr Biochem J. 2016;88(6):70-5. DOI: 10.15407/ubj88.06.070. PMID: 29236285.
Ignarro LJ (ed). Nitric Oxide: Biology and Pathobiology. 2nd Edition. Academic Press; 2010.
Wu G, Bazer FW, Davis TA, Kim SW, Li P, Marc Rhoads J, et al. Arginine metabolism and nutrition in growth, health and disease. Amino Acids. 2009;37(1):153-68. DOI: 10.1007/s00726-008-0210-y. PMID: 19030957.
Wu G, Bazer FW, Satterfield MC, Li X, Wang X, Johnson GA, et al. Impacts of arginine nutrition on embryonic and fetal development in mammals. Amino Acids. 2013;45(2):241-56. DOI: 10.1007/s00726-013-1515-z. PMID: 23732998.
Pautz A, Art J, Hahn S, Nowag S, Voss C, Kleinert H. Regulation of the expression of inducible nitric oxide synthase. Nitric Oxide. 2010;23(2):75-93. DOI: 10.1016/j.niox.2010.04.007. PMID: 20438856.
Wu G, Bazer FW, Davis TA, Kim SW, Li P, Marc Rhoads J, et al. Arginine metabolism and nutrition in growth, health and disease. Amino Acids. 2009;37(1):153-68. DOI: 10.1007/s00726-008-0210-y. PMID: 19030957.
Tsutsui M, Shimokawa H, Otsuji Y, Yanagihara N. Pathophysiological relevance of NO signaling in the cardiovascular system: novel insight from mice lacking all NO synthases. Pharmacol Ther. 2010;128(3):499-508. DOI: 10.1016/j.pharmthera.2010.08.010. PMID: 20826180.
Lapovets L, Lutsik B. Laboratory immunology. Kyiv: Aral; 2004. 173 p. [In Ukrainian].
Nabil H, Moemen LA, Abuelela M. Studying the levels of malondialdehyde and antioxidant parameters in normal and abnormal human seminal plasma. Australian Journal of Basic and Applied Sciences. 2008;2:773-8. Available at: https://is.gd/H5cwFl
Ayala A, Munoz MF, Arguelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014;2014:360438. DOI: 10.1155/2014/360438. PMID: 24999379.
Henkel RR. Leukocytes and oxidative stress: dilemma for sperm function and male fertility. Asian J Androl. 2011;13(1):43-52. DOI: 10.1038/aja.2010.76. PMID: 21076433.
Bryan NS, Bian K, Murad F. Discovery of the nitric oxide signaling pathway and targets for drug development. Front Biosci (Landmark Ed). 2009;14:1-18. DOI: 10.2741/3228. PMID: 19273051.
Siomek A. NF-κB signaling pathway and free radical impact. Acta Biochim Pol. 2012;59(3):323-31. PMID: 22855720.
Ignarro LJ. Nitric oxide, second edition: biology and pathobiology. 2nd ed. N.Y.: Science Press; 2009. 845 p.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.